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DNA digital data storage is the process of encoding and decoding binary data to and from synthesized strands of DNA.[1][2]

While DNA as a storage medium has enormous potential because of its high storage density, its practical use is currently severely limited because of its high cost and very slow read and write times.[3]

In June 2019, scientists reported that all 16 GB of text from Wikipedia's English-language version have been encoded into synthetic DNA.[4]

Cell free

Currently the most wide spread DNA sequencing technology in use is one developed by Illumina which involves immobilization of single stranded DNA on a solid support, polymerase chain reaction (PCR) amplification of the sequences, and labeling of the individual DNA bases with complementary bases tagged with fluorescent markers (see Illumina dye sequencing). The fluorescence pattern (a different color for each of the four DNA bases) can then be captured in an image and processed to determine the DNA sequence.[1] A recently developed alternative is the nanopore technology in which DNA molecules are passed through a nano scale pore under the control of a ratcheting enzyme. The passage of the DNA molecules causes small change in electrical current that can be measured. The main advantage of the nanopore technology is that it can be read in real time.[1] However the read accuracy of this technology is currently insufficient for data storage.[5]

In vivo

The genetic code within living organisms can potentially be co-opted to store information. Furthermore synthetic biology can be used to engineer cells with "molecular recorders" to allow the storage and retrieval information stored in the cell's genetic material.[1] CRISPR gene editing can also be used to insert artificial DNA sequences into the genome of the cell.[1]

History

Vorlage:Primary sources

The idea of DNA digital data storage dates back to 1959, when the physicist Richard P. Feynman, in "There's Plenty of Room at the Bottom: An Invitation to Enter a New Field of Physics" outlined the general prospects for the creation of artificial objects similar to objects of the microcosm (including biological) and having similar or even more extensive capabilities.[6] In 1964-65 Mikhail Samoilovich Neiman, the Soviet physicist, published 3 articles about microminiaturization in electronics at the molecular-atomic level, which independently presented general considerations and some calculations regarding the possibility of recording, storage, and retrieval of information on synthesized DNA and RNA molecules.[7][8][9] After the publication of the first M.S. Neiman's paper and after receiving by Editor the manuscript of his second paper (January, the 8th, 1964, as indicated in that paper) the interview with cybernetician Norbert Wiener was published.[10] N. Wiener expressed ideas about miniaturization of computer memory, close to the ideas, proposed by M. S. Neiman independently. These Wiener's ideas M. S. Neiman mentioned in the third of his papers. This story is described in details.[11]

One of the earliest uses of DNA storage occurred in a 1988 collaboration between artist Joe Davis and researchers from Harvard. The image, stored in a DNA sequence in E.coli, was organized in a 5 x 7 matrix that, once decoded, formed a picture of an ancient Germanic rune representing life and the female Earth. In the matrix, ones corresponded to dark pixels while zeros corresponded to light pixels.[12]

In 2007 a device was created at the University of Arizona using addressing molecules to encode mismatch sites within a DNA strand. These mismatches were then able to be read out by performing a restriction digest, thereby recovering the data.[13]

In 2011, George Church, Sri Kosuri, and Yuan Gao carried out an experiment that would encode a 659-kb book that was co-authored by Church. To do this, the research team did a two-to-one correspondence where a binary zero was represented by either an adenine or cytosine and a binary one was represented by a guanine or thymine. After examination, 22 errors were found in the DNA.[12]

In 2012, George Church and colleagues at Harvard University published an article in which DNA was encoded with digital information that included an HTML draft of a 53,400 word book written by the lead researcher, eleven JPG images and one JavaScript program. Multiple copies for redundancy were added and 5.5 petabits can be stored in each cubic millimeter of DNA.[14] The researchers used a simple code where bits were mapped one-to-one with bases, which had the shortcoming that it led to long runs of the same base, the sequencing of which is error-prone. This result showed that besides its other functions, DNA can also be another type of storage medium such as hard drives and magnetic tapes.[15]

In 2013, an article led by researchers from the European Bioinformatics Institute (EBI) and submitted at around the same time as the paper of Church and colleagues detailed the storage, retrieval, and reproduction of over five million bits of data. All the DNA files reproduced the information between 99.99% and 100% accuracy.[16] The main innovations in this research were the use of an error-correcting encoding scheme to ensure the extremely low data-loss rate, as well as the idea of encoding the data in a series of overlapping short oligonucleotides identifiable through a sequence-based indexing scheme.[15] Also, the sequences of the individual strands of DNA overlapped in such a way that each region of data was repeated four times to avoid errors. Two of these four strands were constructed backwards, also with the goal of eliminating errors.[16] The costs per megabyte were estimated at $12,400 to encode data and $220 for retrieval. However, it was noted that the exponential decrease in DNA synthesis and sequencing costs, if it continues into the future, should make the technology cost-effective for long-term data storage by 2023.[15]

In 2013, a software called DNACloud was developed by Manish K. Gupta and co-workers to encode computer files to their DNA representation. It implements a memory efficiency version of the algorithm proposed by Goldman et al. to encode (and decode) data to DNA (.dnac files).[17][18]

The long-term stability of data encoded in DNA was reported in February 2015, in an article by researchers from ETH Zurich. The team added redundancy via Reed–Solomon error correction coding and by encapsulating the DNA within silica glass spheres via Sol-gel chemistry.[19]

In 2016 research by Church and Technicolor Research and Innovation was published in which, 22 MB of a MPEG compressed movie sequence were stored and recovered from DNA. The recovery of the sequence was found to have zero errors.[20]

In March 2017, Yaniv Erlich and Dina Zielinski of Columbia University and the New York Genome Center published a method known as DNA Fountain that stored data at a density of 215 petabytes per gram of DNA. The technique approaches the Shannon capacity of DNA storage, achieving 85% of the theoretical limit. The method was not ready for large-scale use, as it costs $7000 to synthesize 2 megabytes of data and another $2000 to read it.[21][22][23]

In March 2018, University of Washington and Microsoft published results demonstrating storage and retrieval of approximately 200MB of data. The research also proposed and evaluated a method for random access of data items stored in DNA.[24][25] In March 2019, the same team announced they have demonstrated a fully automated system to encode and decode data in DNA.[26]

Research published by Eurecom and Imperial College in January 2019, demonstrated the ability to store structured data in synthetic DNA. The research showed how to encode structured or, more specifically, relational data in synthetic DNA and also demonstrated how to perform data processing operations (similar to SQL) directly on the DNA as chemical processes.[27][28]

In June 2019, scientists reported that all 16 GB of Wikipedia have been encoded into synthetic DNA.[4]

The first article describing data storage on native DNA sequences via enzymatic nicking was published in April 2020. In the paper, scientists demonstrate a new method of recording information in DNA backbone which enables bit-wise random access and in-memory computing.[29]

Davos Bitcoin Challenge

On January 21, 2015, Nick Goldman from the European Bioinformatics Institute (EBI), one of the original authors of the 2013 Nature paper,[16] announced the Davos Bitcoin Challenge at the World Economic Forum annual meeting in Davos.[30][31] During his presentation, DNA-tubes were handed out to the audience with the message that each tube contained the private key of exactly one bitcoin, all coded in DNA. The first one to sequence and decode the DNA could claim the bitcoin and win the challenge. The challenge was set for three years and would close if nobody claimed the prize before January 21, 2018.[31]

Almost three years later on January 19, 2018, the EBI announced that a Belgian PhD student, Sander Wuyts of the University of Antwerp and Vrije Universiteit Brussel, was the first one to complete the challenge.[32][33] Next to the instructions on how to claim the bitcoin (stored as a plain text and PDF file), the logo of the EBI, the logo of the company that printed the DNA (CustomArray) and a sketch of James Joyce were retrieved from the DNA.[34]

DNA of Things

The concept of the DNA of Things (DoT) was introduced in 2019 by a team of researchers from Israel and Switzerland, including Yaniv Erlich and Robert Grass.[35][36][37] DoT encodes digital data into DNA molecules, which are then embedded into objects. This gives the ability to create objects that carry their own blueprint, similar to biological organisms. In contrast to Internet of things, which is a system of interrelated computing devices, DoT creates objects which are independent storage objects, completely off-grid.

As a proof of concept for DoT, the researcher 3D-printed a Stanford bunny which contains its blueprint in the plastic filament used for printing. By clipping off a tiny bit of the ear of the bunny, they were able to read out the blueprint, multiply it and produce a next generation of bunnies. In addition, the ability of DoT to serve for steganographic purposes was shown by producing non-distinguishable lenses which contain a YouTube video integrated into the material.

See also


Einzelnachweise

  1. ↑ a b c d e Molecular digital data storage using DNA. In: Nature Reviews. Genetics. 20, Nr. 8, August 2019, S. 456–466. Modul:Vorlage:Handle * library URIutil invalid. PMID 31068682.
  2. ↑ Trends to store digital data in DNA: an overview. In: Molecular Biology Reports. 45, Nr. 5, October 2018, S. 1479–1490. Modul:Vorlage:Handle * library URIutil invalid. PMID 30073589.
  3. ↑ DNA as a digital information storage device: hope or hype?. In: 3 Biotech. 8, Nr. 5, May 2018, S. 239. Modul:Vorlage:Handle * library URIutil invalid. PMID 29744271. PMC 5935598 (freier Volltext).
  4. ↑ a b Stephen Shankland: Startup packs all 16GB of Wikipedia onto DNA strands to demonstrate new storage tech - Biological molecules will last a lot longer than the latest computer storage technology, Catalog believes.. In: CNET, 29 June 2019. Abgerufen im 7 August 2019. 
  5. ↑ Three decades of nanopore sequencing. In: Nature Biotechnology. 34, Nr. 5, May 2016, S. 518–24. Modul:Vorlage:Handle * library URIutil invalid. PMID 27153285. PMC 6733523 (freier Volltext).
  6. ↑ Richard P. Feynman: There's Plenty of Room at the Bottom. In: Annual meeting of the American Physical Society. 29 December 1959.
  7. ↑ Some fundamental issues of microminiaturization. In: Radiotekhnika. Nr. 1, 1964, S. 3–12 (in Russ.).
  8. ↑ On the relationships between the reliability, performance and degree of microminiaturisation at the molecular-atomic level.. In: Radiotekhnika. Nr. 1, 1965, S. 1–9 (in Russ.).
  9. ↑ On the molecular memory systems and the directed mutations.. In: Radiotekhnika. Nr. 6, 1965, S. 1–8 (in Russ.).
  10. ↑ Interview: machines smarter than men?. In: US News & World Report. 56, 1964, S. 84–86.
  11. ↑ Storage devices based on artificial DNA: the birth of an idea and the first publications.. In: Voprosy istorii estestvoznaniia i tekhniki. 41, Nr. 4, 2020, S. 666–76 ((in Russ.). Modul:Vorlage:Handle * library URIutil invalid.
  12. ↑ a b How DNA could store all the world's data. In: Nature. 537, Nr. 7618, September 2016, S. 22–4. bibcode:2016Natur.537...22E. Modul:Vorlage:Handle * library URIutil invalid. PMID 27582204.
  13. ↑ Biocompatible Writing of Data into DNA. In: Journal of Bionanoscience. 1, Nr. 1, 1. Juni 2007, S. 17–21. arxiv:1708.08027. Modul:Vorlage:Handle * library URIutil invalid.
  14. ↑ Next-generation digital information storage in DNA. In: Science. 337, Nr. 6102, September 2012, S. 1628. bibcode:2012Sci...337.1628C. Modul:Vorlage:Handle * library URIutil invalid. PMID 22903519.
  15. ↑ a b c Synthetic double-helix faithfully stores Shakespeare's sonnets. In: Nature. 2013. Modul:Vorlage:Handle * library URIutil invalid.
  16. ↑ a b c Towards practical, high-capacity, low-maintenance information storage in synthesized DNA. In: Nature. 494, Nr. 7435, February 2013, S. 77–80. bibcode:2013Natur.494...77G. Modul:Vorlage:Handle * library URIutil invalid. PMID 23354052. PMC 3672958 (freier Volltext).
  17. ↑ Vorlage:Cite arxiv
  18. ↑ On optimal family of codes for archival DNA storage 25 April 2016, ISBN 978-1-4673-8308-0, S. 123–127, arxiv:1501.07133, Modul:Vorlage:Handle * library URIutil invalid.
  19. ↑ Robust chemical preservation of digital information on DNA in silica with error-correcting codes. In: Angewandte Chemie. 54, Nr. 8, February 2015, S. 2552–5. Modul:Vorlage:Handle * library URIutil invalid. PMID 25650567.
  20. ↑ Forward Error Correction for DNA Data Storage. In: Procedia Computer Science. 80, 2016, S. 1011–1022. Modul:Vorlage:Handle * library URIutil invalid.
  21. ↑ Ed Yong: This Speck of DNA Contains a Movie, a Computer Virus, and an Amazon Gift Card. In: The Atlantic. Abgerufen im 3 March 2017. 
  22. ↑ DNA could store all of the world's data in one room. In: Science Magazine . 2 March 2017. Abgerufen im 3 March 2017.
  23. ↑ DNA Fountain enables a robust and efficient storage architecture. In: Science. 355, Nr. 6328, March 2017, S. 950–954. bibcode:2017Sci...355..950E. Modul:Vorlage:Handle * library URIutil invalid. PMID 28254941.
  24. ↑ Random access in large-scale DNA data storage. In: Nature Biotechnology. 36, Nr. 3, March 2018, S. 242–248. Modul:Vorlage:Handle * library URIutil invalid. PMID 29457795.
  25. ↑ Prachi Patel: DNA Data Storage Gets Random Access. In: IEEE Spectrum: Technology, Engineering, and Science News . 20. Februar 2018. Abgerufen am 8. September 2018.
  26. ↑ Microsoft, UW demonstrate first fully automated DNA data storage (Amerikanisches Englisch) In: Innovation Stories . 21. März 2019. Abgerufen am 21. März 2019.
  27. ↑ OligoArchive: Using DNA in the DBMS storage hierarchy. In: Conference on Innovative Data Systems Research (CIDR). 2019.
  28. ↑ OligoArchive Website (Amerikanisches Englisch) In: oligoarchive.github.io . Abgerufen am 6. Februar 2019.
  29. ↑ S. Kasra Tabatabaei, Boya Wang, Nagendra Bala Murali Athreya, Behnam Enghiad, Alvaro Gonzalo Hernandez, Christopher J. Fields, Jean-Pierre Leburton, David Soloveichik, Huimin Zhao, Olgica Milenkovic: DNA punch cards for storing data on native DNA sequences via enzymatic nicking. In: Nature Communications. 11, Nr. 1, 8 April 2020, S. 1–10. Modul:Vorlage:Handle * library URIutil invalid. PMID 32269230. PMC 7142088 (freier Volltext).
  30. ↑ World Economic Forum (2015-03-10) Future Computing: DNA Hard Drives | Nick Goldman[1]
  31. ↑ a b DNA storage | European Bioinformatics Institute (Englisch) In: www.ebi.ac.uk . Abgerufen am 19. Mai 2018.
  32. ↑ Belgian PhD student decodes DNA and wins a Bitcoin | European Bioinformatics Institute (Englisch) In: www.ebi.ac.uk . Abgerufen am 19. Mai 2018.
  33. ↑ A Piece of DNA Contained the Key to 1 Bitcoin and This Guy Cracked the Code (en-us). In: Motherboard, 24. Januar 2018. Abgerufen am 19. Mai 2018. 
  34. ↑ From DNA to bitcoin: How I won the Davos DNA-storage Bitcoin Challenge (en-US). In: Sander Wuyts, 16. Januar 2018. Abgerufen am 19. Mai 2018. 
  35. ↑ Koch, Julian: A DNA-of-things storage architecture to create materials with embedded memory. In: Nature Biotechnology. 38, Nr. 1, 2019, S. 39–43. Modul:Vorlage:Handle * library URIutil invalid. PMID 31819259.
  36. ↑ Megan Molteni: These Plastic Bunnies Got a DNA Upgrade. Next up, the World?. In: Wired, 9. Dezember 2019. 
  37. ↑ Robert Lee Hotz: Scientists Store Data in Synthetic DNA Embedded in a Plastic Bunny. In: Wall Street Journal, 9. Dezember 2019. 

Further reading

Vorlage:Refbegin

Vorlage:Refend

[[Category:DNA]] [[Category:Molecular biology]] [[Category:Storage media]] [[Category:Computational biology]]

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